determination of the optimal enrichment artemia franciscana with a synbiotic combination of probiotics pediococcus acidilactici and prebiotic fructooligosaccharide

in this study the optimal amount of enriching artemia franciscana with a synbiotic combination of pediococcus acidilactici and fructooligosaccharide (fos( was investigated. the experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics p. acidilactici (1×109, 1×108 and 1× 107 cfu per l) and multi-level enriched prebiotic fos (1, 2 and 5) g per l of solution and control. to evaluate the enrichment of adult artemia with each treatment, sampling was performed at 2, 4 and 6 hr after initiation of enrichment. the results showed that a synbiotic containing a probiotic treatments p. acidilactici at 1 × 109 and 1 × 108 cfu per l had more bacteria than a synbiotic containing a probiotic treatment with 1 × 107 cfu per l (p < 0.05), but did not show significant difference between levels of 1 × 109 and 1 × 108 cfu per l (p > 0.05). the highest number of attached bacteria (6.78 ± 0.03 log cfu g-1) to adult artemia was shown after 6 hr of enrichment that showed significant difference with 2 hr, but did not show significant difference with 4 hr time. in conclusion, the results of this study showed that adult artemia in a short time (about 4 hr) unlike nauplii artemia can retain a large amount of probiotic (1 × 108 cfu p. acidilactici per l and 5 g per l fos prebiotic) on their own.